The article discusses the development of book printing in the Czech in the 16th – early 17th centuries, two periods in the development of Czech book printing are distinguished: 1501–1547 and after the events of 1547 until the Battle of White Mountain. The author reveals the changes that have taken place in the working conditions of printing houses, the attitude of the royal authorities to book printing, the repertoire of Czech printed books, the number of published products.